Merck 105394 0500 Mossel Broth Enterobacteriaceae Enrichment Broth Acc. To Mossel For Microbiology 500 GR.

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Description
Catalogue Number	105394
Synonyms	MOSSEL Enterobacteriaceae enrichment broth, Enterobacteriaceae enrichment broth
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

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Product Information
HS Code	3821 00 00
Quality Level	MQ200

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Physicochemical Information
pH value	7.3 (45 g/l, H₂O, 37 °C) (after autoclaving)
Bulk density	630 kg/m3
Solubility	45 g/l

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Safety Information according to GHS
Hazard Statement(s)	H412: Harmful to aquatic life with long lasting effects.
Precautionary Statement(s)	P273: Avoid release to the environment.\nP501: Dispose of contents/ container to an approved waste disposal plant.
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3\nRelatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Appearance (clearness)	clear
Appearance (color)	green
pH-value (25 °C)	7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.\nMixed inoculum E.coli resp. Salmonella and E.faecalis.\nAdditionaly monoculture of E.faecalis\nConfirmation on VRBD agar resp. Tryptic soy agar
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))	≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))	≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))	≥ 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087))	≥ 10000
Growth on VRBD-Agar(Escherichia coli ATCC 8739 (WDCM 00012))	> 10 colonies
Growth on VRBD-Agar(Escherichia coli ATCC 25922 (WDCM 00013))	> 10 colonies
Growth on VRBD-Agar(Salmonella typhimurium ATCC 14028 (WDCM 00031))	> 10 colonies
Growth on VRBD-Agar(Salmonella enteritidis ATCC 13076 (WDCM 00030))	> 10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009))	total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087))	total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012))	pink to red colonies with or without precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013))	pink to red colonies with or without precipitation halo
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031))	pink to red colonies with or without precipitation halo
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030))	pink to red colonies with or without precipitation halo
Incubation:\nEE broth: 24 ± 2 hours at 37 ± 1 °C aerobic\nConfirmation on VRBD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic