Merck 100416 0500 R2A Agar For Microbiology 500 GR.

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Description
Catalogue Number	100416

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Product Information
HS Code	3821 00 00
Quality Level	MQ200

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Physicochemical Information
Solubility	18.2 g/l

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Safety Information according to GHS
Hazard Pictogram(s)	\n
Hazard Statement(s)	H317: May cause an allergic skin reaction.
Precautionary Statement(s)	P261: Avoid breathing dust.\nP272: Contaminated work clothing should not be allowed out of the workplace.\nP280: Wear protective gloves.\nP302 + P352: IF ON SKIN: Wash with plenty of water.\nP333 + P313: If skin irritation or rash occurs: Get medical advice/ attention.\nP362 + P364: Take off contaminated clothing and wash it before reuse.
Signal Word	Warning
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3\nRelatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Appearance (clearness)	clear to slightly turbid
Appearance (color)	yellowish
pH-value (25 °C)	7.0 - 7.4
Typical composition (g/l): Yeast extract 0.5; Proteose Peptone 0.5; Casein hydrolysate 0.5; Glucose 0.5; Starch soluble 0.5; Sodium pyruvate 0.3; di-Potassium hydrogen phosphate 0.3; Magnesium sulfate anhydrous 0.024; Agar-agar 15.0.
Growth promotion test in accordance with the European Pharmacopeia.
Inoculum 35°C / 24h (Escherichia coli ATCC 8739 (WDCM 00012))	10 - 100
Inoculum 35°C / 24h (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	10 - 100
Inoculum 35°C / 24h (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	10 - 100
Inoculum 35°C / 24h (Staphylococcus aureus ATCC 6538 (WDCM 00032))	10 - 100
Inoculum 35°C / 24h (Bacillus subtilis ATCC 6633 (WDCM 00003))	10 - 100
Colony count 35°C/24 h (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count 35°C/24 h (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
Colony count 35°C/24 h (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))
Colony count 35°C/24 h (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Colony count 35°C/24 h (Bacillus subtilis ATCC 6633 (WDCM 00003))
Recovery rate 35 °C/24 h (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 70 %
Recovery rate 35 °C/24 h (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	≥ 70 %
Recovery rate 35 °C/24 h (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	≥ 70 %
Recovery rate 35 °C/24 h (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≥ 70 %
Recovery rate 35 °C/24 h (Bacillus subtilis ATCC 6633 (WDCM 00003))	≥ 70 %
Inoculum 20-25°C / 72h (Escherichia coli ATCC 8739 (WDCM 00012))	10 - 100
Inoculum 20-25°C / 72h (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	10 - 100
Inoculum 20-25°C / 72h (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	10 - 100
Inoculum 20-25°C / 72h (Staphylococcus aureus ATCC 6538 (WDCM 00032))	10 - 100
Inoculum 20-25°C / 72h (Bacillus subtilis ATCC 6633 (WDCM 00003))	10 - 100
Inoculum 20-25°C / 72h (Pseudomonas fluorescens ATCC 17386)	10 - 100
Inoculum 20-25°C / 72h (Methylobacterium extorquens NBRC 15911)	10 - 100
Colony count 20-25°C/72 h (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count 20-25°C/72 h (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
Colony count 20-25°C/72 h (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))
Colony count 20-25°C/72 h (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Colony count 20-25°C/72 h (Bacillus subtilis ATCC 6633 (WDCM 00003))
Colony count 20-25°C/72 h (Pseudomonas fluorescens ATCC 17386)
Colony count 20-25°C/72 h (Methylobacterium extorquens NBRC 15911)
Recovery rate 20-25 °C/72 h (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 70 %
Recovery rate 20-25 °C/72 h (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	≥ 70 %
Recovery rate 20-25 °C/72 h (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	≥ 70 %
Recovery rate 20-25 °C/72 h (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≥ 70 %
Recovery rate 20-25 °C/72 h (Bacillus subtilis ATCC 6633 (WDCM 00003))	≥ 70 %
Recovery rate 20-25 °C/72 h (Pseudomonas fluorescens ATCC 17386)	≥ 50 %
Recovery rate 20-25 °C/72 h (Methylobacterium extorquens NBRC 15911)	≥ 50 %
Incubation: 24 hrs.; 35°C; aerobic and 72 hrs.; 20-25 °C; aerobic