Merck 110398 0500 Fraser Listeria Selective Enrichment Broth (Base) For Microbiology 500 GR.

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Description
Catalogue Number	110398
Replaces	1.10398
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

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Product Information
HS Code	3821 00 00
Quality Level	MQ200

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Physicochemical Information
pH value	7.0 - 7.4 (57.4 g/l, H₂O, 25 °C)
Solubility	57.4 g/l

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Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3\nRelatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Appearance (clearness)	clear to almost clear
Appearance (color)	yellowish-brown
pH-value (25 °C)	7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.\nMixed inoculum of Listeria, E.coli and E.faecalis\nAdditionaly monoculture of E.coli and E.faecalis\nConfirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))	≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))	≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087))	≥ 1000
Blacking (Listeria monocytogenes ATCC 13932 (WDCM 00021))	+
Blacking (Listeria monocytogenes ATCC 35152 (WDCM 00109))	+
Blacking (Escherichia coli ATCC 25922 (WDCM 00013))	-
Blacking (Escherichia coli ATCC 8739 (WDCM 00012))	-
Blacking (Enterococcus faecalis ATCC 19433 (WDCM 00009))	-
Blacking (Enterococcus faecalis ATCC 29212 (WDCM 00087))	-
Growth on ALOA® medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))	> 10 colonies
Growth on ALOA® medium(Listeria monocytogenes ATCC 35152 (WDCM 00109))	> 10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021))	blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109))	blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))	total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))	total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087))	< 100 colonien
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009))	< 100 colonies
Incubation:\nHalf-Fraser Broth: 24 ± 2 hours at 30 ± 1°C\nFraser Broth: 48 ± 2 hours at 37 ± 1°C\nConfirmation on ALOA® medium: 44 ± 4 hours at 37 ±1°C\nConfirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C