Merck 109261 1000 Lugol'S Solution (Diluted iodine-Potassium iodide Solution) For The Gram Staining Method 1 LT.

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Description
Catalogue Number	109261
Synonyms	Iodine-potassium iodide solution
Description	Lugol's solution (diluted iodine-potassium iodide solution)
Overview	Lugol’s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Cat.no. 109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step (Gram's decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Cat.no. 109217) and will then appear pink to red.\nLugol's solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

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Product Information
HS Code	3212 90 00
Quality Level	MQ400

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Applications
Application	for the Gram staining method

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Physicochemical Information
Boiling point	100 °C (1013 hPa)
Density	1.01 g/cm3 (20 °C)
pH value	3.5 (H₂O, 20 °C)

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Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 2 obviously hazardous to water

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Suitability for microscopy	passes test
gram-positve microorganisms	blue-violet
gram-negative microorganisms	pink to red

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