Merck 109205 0500 Mtsb-Broth With Novobiocin For Microbiology 500 GR.

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Description
Catalogue Number	109205

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Product Information
HS Code	3821 00 00
Quality Level	MQ200

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Physicochemical Information
pH value	7.1 - 7.5 (33 g/l, H₂O, 25 °C) (after autoclaving)
Solubility	33 g/l

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Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3\nRelatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Appearance (clearness)	clear
Appearance (color)	yellowish-brown
pH-value (25 °C)	7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.\nMixed inoculum of E.coli and S.aureus\nAdditionaly monoculture of S.aureus
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	≥ 10000
Growth (Escherichia coli ATCC 35150 (O157))	> 10 yellowish-brown colonies on CT-SMAC agar
Growth (Escherichia coli ATCC 700728 (WDCM 00014))	> 10 yellowish-brown colonies on CT-SMAC agar
Growth Escherichia coli ATCC 25922 (WDCM 00013)	> 10 pink colonies on CT-SMAC agar
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034))	total inhibition on TSA
Incubation:\nmTSB broth: 18 - 24 hours at 41,5 °C, aerobic\nConfirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic