Merck 107620 0500 Pseudomonas Selective Agar (Base) For Microbiology 500 GR.

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Description
Catalogue Number	107620
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

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Product Information
HS Code	3821 00 00
Quality Level	MQ200

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Physicochemical Information
pH value	6.9 - 7.3 (48.4 g/l, H₂O, 25 °C)
Solubility	48.4 g/l

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Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Base tested with Pseudomonas CN selektiv supplement art. no. 1.07624 and Pseudomonas CFC selektiv supplement art. no. 1.07267.
Appearance (clearness)	clear
Appearance (color)	yellowish
pH-value (25 °C)	6.9 - 7.3
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Growth promotion test as CN-Agar
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024))
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
Colony count (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024))
Colony count (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))
Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
Recovery on test medium (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024))	≥ 50 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	≥ 50 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	≥ 50 %
Colonies (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024))	Blue-green colonies with fluorescence
Colonies (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	blue-green colonies with fluorescence
Colonies (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	blue-green colonies with fluorescence
Growth (Escherichia coli ATCC 8739 (WDCM 00012))	total inhibition
Growth Escherichia coli ATCC 25922 (WDCM 00013)	total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))	total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087))	total inhibition
Incubation: 44 ± 4 hours at 36 ± 2 °C aerobic
Growth promotion test as CFC-Agar
Inoculum on reference medium (Pseudomonas fluorescens ATCC 13525(WDCM00115))
Inoculum on reference medium (Pseudomonas fragi ATCC 4973 (WDCM00116))
Colony count (Pseudomonas fluorescens ATCC 13525 (WDCM00115))
Colony count (Pseudomonas fragi ATCC 4973 (WDCM00116))
Recovery on test medium (Pseudomonas fluorescens ATCC 13525 (WDCM 00115))	≥ 50 %
Recovery on test medium (Pseudomonas fragi ATCC 4973 (WDCM 00116))	≥ 50 %
Colonies (Pseudomonas fluorescens ATCC 13525 (WDCM 00115))	greenish
Colonies (Pseudomonas fragi ATCC 4973 (WDCM 00116))	milky
Growth(Escherichia coli ATCC 8739 (WDCM 00012))	total inhibition
Growth (Escherichia coli ATCC 25922 (WDCM 00013))	total inhibition
Incubation: 44 ± 4 °C hours at 25 ± 1 °C aerobic\nA recovery rate of 50 % is equivalent to a productivity value of 0.5.\nThe indicated colony counts result from the sum of a triple determination.\nReference media: Tryptic Soy Agar