RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.
Aluminum TLC plates, RP-18 modified silica gel coated with flourescent indicator F254s. The RP-18 modified silica gel TLC plates come in a quantity of 20 size 20x20 cm for thin layer chromatography applications.
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Safety Information according to GHS
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Storage class
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10 - 13 Other liquids and solids
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Storage and Shipping Information
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Storage
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Store at +2°C to +30°C.
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Specifications
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Specific surface area (according to BET; 5-Pt. measurement)
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480 - 540 m²/g
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Pore volume (N₂-isotherm)
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0.74 - 0.84 ml/g
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d 50 (laser diffraction, size distribution)
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9.5 - 11.5 µm
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Layer thickness
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160 - 200 µm
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Deviation of layer thickness per plate
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≤ 30 µm
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Chromatographic testings:\nA) Cholesterin test
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hRf-values\n- cholesterin, cholesterin test
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32 - 39
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B) Steroid test
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- methyltestosterone, steroid test
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14 - 22
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- Reichstein S, steroid test
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26 - 36
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- hydrocortisone, steroid test
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36 - 46
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Typical value determined on an activated sheet (120°C, 15 min)\nEluent a) aceton/ water (95/5 v/v)\nEluent b) aceton/ water (60/40 v/v)