Merck 105463 0500 Plate Count Agar acc. ISO 4833, ISO 17410 and FDA-BAM GranuCult® 500 GR.

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Description
Catalogue Number	105463
Replaces	1.05463.5007
Synonyms	Casein-peptone dextrose yeast agar
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

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Product Information
HS Code	3821 00 00
Quality Level	MQ200

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Physicochemical Information
pH value	7.0 (22 g/l, H₂O, 30 °C) (after autoclaving)
Bulk density	560 kg/m3
Solubility	22.5 g/l

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Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3\nRelatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Appearance (clearness)	clear
Appearance (color)	yellowish to yellowish-brown
Solidification behaviour (2 hrs., 40 °C)	liquid
Solidification behaviour (4 hrs., 45 °C)	liquid
pH-value (25 °C)	6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Incubation: 72 ± 3 hours at 30 ± 1 °C; aerobic
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))
Inoculum on reference medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))	10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19118)	10 - 100
Inoculum on reference medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))	10 - 100
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003))
Colony count (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))
Colony count (Listeria monocytogenes ATCC 19118)
Colony count (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≥ 70 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 70 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003))	≥ 70 %
Recovery on test medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))	≥ 70 %
Recovery on test medium (Listeria monocytogenes ATCC 19118)	≥ 70 %
Recovery on test medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))	≥ 70 %
Incubation: 24 hours at 35 - 37 °C; aerobic
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003))
Recovery (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≥ 70 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 70 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003))	≥ 70 %
Incubation: up to 10 days at 6,5 ± 1 °C, aerobic
Inoculum on reference medium (Pseudomonas fluorescens ATCC 13525(WDCM00115))
Inoculum on reference medium(Yersinia enterocolitica DSM 13030 (WDCM 00216))
Colony count (Pseudomonas fluorescens ATCC 13525 (WDCM00115))
Colony count (Yersinia enterocolitica DSM 13030 (WDCM 00216))
Recovery on test medium (Pseudomonas fluorescens ATCC 13525 (WDCM 00115))	≥ 70 %
Recovery on test medium(Yersinia enterocolitica DSM 13030 (WDCM 00216))	≥ 70 %
Reference: Tryptic soy agar.\nA recovery rate of 70 % is equivalent to a productivity value of 0.7.\nThe indicated colony counts result from the sum of a triple determination.