Merck 105459 0500 Tryptic Soy Broth Casein-Peptone Soymeal-Peptone Broth For Microbiology Usp 500 GR.

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Description
Catalogue Number	105459
Replaces	1054599027; 1.05459.5007
Synonyms	Casein-peptone soymeal-peptone broth, Casein-peptone soyameal-peptone broth

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Product Information
HS Code	3821 00 00
Quality Level	MQ200

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Physicochemical Information
Bulk density	570 kg/m3
Solubility	30 g/l

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Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3\nRelatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Appearance (clearness)	clear
Appearance (color)	yellowish brown
pH-value (25 °C)	7.1 - 7.5
Stability test (7 days, room temperature)	clear
Typical composition (g/litre): Pepton from casein 17.0; Pepton from soymeal 3.0; D(+)-Glucose monohydrate 2.5; Sodium chloride 5.0; di-Potassium hydrogen phosphate 2.5.
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))	10 - 100
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))	10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	10 - 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	10 - 100
Inoculum on reference medium (Streptococcus pneumoniae ATCC 6301)	10 - 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))	10 - 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	10 - 100
Inoculum on reference medium (Staphylococcus epidermidis ATCC 12228 (WDCM 00036))	10 - 100
Inoculum on reference medium (Candida albicans ATCC 2091 (WDCM 00055))	10 - 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054))	10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))	10 - 100
reference medium: Tryptic Soy Agar (Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus epidermidis), SABOURAUD-2%-Glucose Agar (Candida albicans, Asperigillus brasiliensis)
Growth / 18-24h at 30-35°C (Staphylococcus aureus ATCC 6538(WDCM 00032))	visible growth
Growth / 18-24h at 30-35°C (Bacillus subtilis ATCC 6633 (WDCM 00003))	visible growth
Growth / 18-24h at 30-35°C (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))	visible growth
Growth / 18-24h at 30-35°C (Escherichia coli ATCC 8739 (WDCM 00012))	visible growth
Growth / 18-24h at 30-35°C (Streptococcus pneumoniae ATCC 6301)	visible growth
Growth / 18-24h at 30-35°C (Salmonella typhimurium ATCC 14028 (WDCM 00031))	visible growth
Growth / 3 days at 20-25°C (Staphylococcus aureus ATCC 6538 (WDCM 00032))	visible growth
Growth / 3 days at 20-25°C (Staphylococcus aureus ATCC 25923 (WDCM 00034))	visible growth
Growth / 3 days at 20-25°C (Staphylococcus epidermidis ATCC 12228 (WDCM 00036))	visible growth
Growth / 3 days at 20-25°C (Bacillus subtilis ATCC 6633 (WDCM 00003))	visible growth
Growth / 5 days at 20-25°C (Candida albicans ATCC 2091 (WDCM 00055))	visible growth
Growth / 5 days at 20-25°C (Candida albicans ATCC 10231 (WDCM 00054))	visible growth
Growth / 5 days at 20-25°C (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053))	visible growth
The casein used for processing the Pepton from casein as contained is beeing sourced from healthy cows and declared good for human consumption. The casein is acid precipitated. Therefor, this product does not come under the scope of Ph.Eur. 5.2.8.\nFor details, please request our BSE-certificate.