Merck 100092 0010 Fraser Listerya Ammonium Iron (III) Supplement (1 Kutu,10 Adet).

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Description
Catalogue Number	100092
Replaces	1000920017

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Product Information
CAS number	1185-57-5
EC number	214-686-6
Hill Formula	C₆H₈O₇*xFe*xH₃N
HS Code	2918 15 00
Quality Level	MQ200

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Applications
Application	FRASER Listeria Ammonium iron(III) Supplement. CAS 1185-57-5, pH 6 - 8 (100 g/l, H₂O, 20 °C).

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Physicochemical Information
Density	1.064 g/cm3 (978 hPa, 26 °C)
pH value	6 - 8 (100 g/l, H₂O, 20 °C)
Solubility	1200 g/l

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Toxicological Information
LD 50 oral	LD50 Rat > 2000 mg/kg

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Safety Information according to GHS
RTECS	GE7540000
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3\nRelatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

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Storage and Shipping Information
Storage	Store at +15°C to +25°C.

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Specifications
Growth promotion test according to ISO 11133\nMixed inoculum of Listeria, E.coli and E.faecalis\nAdditionaly monoculture of E.coli and E.faecalis\nPre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth\nConfirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium\n- Listeria monocytogenes ATCC 19111 (WDCM 00020)	10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013)	≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012)	≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	≥ 1000
Blacking\n- Listeria monocytogenes ATCC 19111 (WDCM 00020)	+
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	+
- Escherichia coli ATCC 25922 (WDCM 00013)	-
- Escherichia coli ATCC 8739 (WDCM 00012)	-
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	-
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	-
Growth on ALOA® medium\n- Listeria monocytogenes ATCC 19111 (WDCM 00020)	> 10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	> 10 CFU
Colonies\n- Listeria monocytogenes ATCC 19111 (WDCM 00020)	bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	bluegreen with non transparent zone
Growth on Tryptic soy agar\n- Escherichia coli ATCC 25922 (WDCM 00013)	no growth
- Escherichia coli ATCC 8739 (WDCM 00012)	no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	< 100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	< 100 CFU
Incubation:\nHalf Fraser Broth: 24 hours at 30°C, aerobic\nFraser Broth: 48 hours at 37°C, aerobic\nConfirmation on ALOA®-Medium: 48 hours at 37°C, aerobic\nConfirmation on Tryptic soy agar: 24 hours at 35°C, aerobic